MILAN — Genoa goalkeeper Mattia Perin pulled out of the Italy squad with injury on Sunday and was replaced by Lazio's Federico Marchetti.
Perin was forced off early in the second half of Genoa's 1-0 defeat at Juventus on Sunday after injuring his right shoulder.
Italy travels to Bulgaria for a qualifying match for the 2016 European Championship on March 28, before hosting England in a friendly at the Juventus Stadium three days later.
Antonio Conte's side is behind Croatia on goal difference at the top of Group H, with both sides having won three matches and drawn one.
Eder and Vazquez grew up in Brazil and Argentina respectively but have Italian passports.
Juventus defender Andrea Barzagli is also included for the first time since the World Cup, after recovering from a lengthy injury.
Midfielders: Luca Antonelli (AC Milan), Andrea Bertolacci (Genoa), Antonio Candreva (Lazio), Alessio Cerci (AC Milan), Matteo Darmian (Torino), Alessandro Florenzi (Roma), Claudio Marchisio (Juventus), Marco Parolo (Lazio), Manuel Pasqual (Fiorentina), Roberto Soriano (Sampdoria), Mirko Valdifiori (Empoli), Marco Verratti (Paris Saint-Germain).
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