Bryant singled home Kyle Schwarber in the top of the fifth to give the Cubs a 4-1 lead and he tried for second after the Brewers threw home.
The play was cut off and the Brewers tried to get Bryant at second. Shortstop Jean Segura appeared to tag Bryant on his helmet, before Bryant's neck or upper back appeared to hit Segura's leg on the slide.
A safe call was upheld following a Brewers replay challenge. Bryant was removed from the game for pinch-runner Jonathan Herrera.
Bryant is hitting .244 with 14 homers and 60 RBIs.